Yr. 2 IHSFC Voucher - Generate a Tll1-flox transgenic mouse

Abstract

We received 2 clones from EuMMCR, performed several rounds of injections, but couldn’t obtain a good male chimera. The only chimera we got was a low percent female that usually don’t produce germline.

We subcloned these clones in presence of a special medium that helps ES cells retain totipotency and performed karyotyping to confirm that the subclones don’t have any abnormalities. We then injected two best subclones and obtained 3 male chimeras with 90-95% coat contribution from the ES cells. The chimeras were set up to breed with B6N females and one of them produced germline heterozygous. More litters will be screened in the nearest future.